Coding

Part:BBa_K5216000:Design

Designed by: Huijia Li   Group: iGEM24_OTIA-Hangzhou   (2024-08-21)


AgaA (β-agarase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1129
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 339
    Illegal AgeI site found at 577
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 384
    Illegal BsaI site found at 985
    Illegal SapI site found at 89
    Illegal SapI site found at 221
    Illegal SapI site found at 284
    Illegal SapI.rc site found at 1066


Design Notes

This β-agarase codon optimization was performed based on the expression strain E.coli.


Source

Gene sequence from Marinimicrobium sp.H1

References